First released: 23rd January 2010
About
- 1.Cold Open: Professor Layton's Theme
- 2.Prologue to the Adventure: Puzzles
- 3.Travel Guide: Descole's Theme
- 4.Compensation 1: Detragigant's Theme
- 5.Departure to the Voyage: Descole's Theme
- 6.Detragan's Echoes: Whistler's Theme
- 7.Rules for the Survivors: Tense Atmosphere
- 8.Puzzles Number 001: Puzzles Reinvented
- 9.Compensation 2: Detragigant's Theme
- 10.Puzzles Number 002: More Puzzles
- 11.Melina's Tenacity: An Uneasy Atmosphere
- 12.People of the Past: The Looming Tower
- 13.The Right Crown: Descole's Theme
- 14.At London: About Town
- 15.The Passionate Whistler: Whistler's Theme
- 16.The Legendary Kingdom: Theme of Ambrosia
- 17.Rest: A Moment of Peace
- 18.A Pursuer Approaching 1: A Pursuer Approaching
- 19.Puzzles Number 003: Revolutionary Idea
- 20.Adjusting the Pace: Pursuit in the Night
- 21.Compensation 3: Detragigant's Theme
- 22.Escape! Professor Layton's Theme
- 23.Puzzles Number 004: The Plot Thickens!
- 24.Enter Descole: Descole's Theme
- 25.Professor Layton's Piano: Song of the Sea
- 26.A Pursuer Approaching 2: A Pursuer Approaching
- 27.Emmy's Efforts: Emmy's Theme
- 28.Whistler's Experiment: Dangerous Experiment
- 29.The Puzzle Explained! Professor Layton's Theme
- 30.Great Conspiracy: Descole's and Ambrosia's Theme
- 31.Overture to Destruction: Descole's Theme
- 32.Detragigant Appears: Detragigant's Theme
- 33.Janice' Crisis: Tense Decision
- 34.Future British Gentleman: Luke's Theme
- 35.The Last Battle: Time of Conclusion
- 36.The Dream Collapses: Theme of Ambrosia
- 37.Father's Memories: Whistler's Theme
- 38.The Feelings Will Always Be Close: Whistler's Theme
- 39.The Eternal Diva / Janice Quatlane (CV Nana Mizuki)
